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Corning Life Sciences polycarbonate erlenmeyer flask with vent cap
Polycarbonate Erlenmeyer Flask With Vent Cap, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polycarbonate+erlenmeyer+flasks+with+vent+cap/polycarbonate+erlenmeyer+flasks+with+vent+cap/pmc12117386-18-0-0
Average 90 stars, based on 1 article reviews
polycarbonate erlenmeyer flask with vent cap - by Bioz Stars, 2026-10
90/100 stars

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Amplification:

Article Title: VASH1/2 inhibition accelerates functional recovery of injured nerves
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (10 6 cells/ml) in Sf-900 III SFM Medium supplemented with 12.5 U/ml penicillin/streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27°C and 110 rpm for 4 days. ..

Article Title: Human retinal ganglion cell axon regeneration by recapitulating developmental mechanisms: effects of recruitment of the mTOR pathway
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (106 cells/ml) in Sf-900 III SFM Medium supplemented with 12.5 U/ml penicillin/streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27°C and 130 rpm for 2-3 days. .. Amplified viruses were purified and concentrated by ultracentrifugation of 27 ml virus-containing supernatant underlaid with 2.7 ml sucrose solution (25% sucrose with 5 mM NaCl and 10 mM EDTA in H 2 O) in OptiSeal polypropylene tubes (Beckmann Coulter) at 80,000 g and 4°C for 80 min.

Article Title: Targeting Vasohibins to Promote Axon Regeneration
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher Scientific) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (106 cells/ml) in Sf-900 III SFM Medium (Thermo Fisher Scientific) supplemented with 12.5 U/ml penicillin/ streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27°C and 110 rpm for 4 d. Baculovirus preparations were pretested on AAV293 cells overnight by adding 1% (v/v) virus. ..

Article Title: Highly efficient transduction of primary adult CNS and PNS neurons
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (10 6 cells/ml) in Sf-900 III SFM Medium supplemented with 12.5 U/ml penicillin/streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27 °C and 130 rpm for 2–3 days. .. Amplified viruses were purified and concentrated by ultracentrifugation of 27 ml virus-containing supernatant underlayed with 2.7 ml sucrose solution (25% sucrose with 5 mM NaCl and 10 mM EDTA in H 2 O) in OptiSeal polypropylene tubes (Beckmann Coulter) at 80,000 g and 4 °C for 80 min.

Suspension:

Article Title: VASH1/2 inhibition accelerates functional recovery of injured nerves
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (10 6 cells/ml) in Sf-900 III SFM Medium supplemented with 12.5 U/ml penicillin/streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27°C and 110 rpm for 4 days. ..

Article Title: Human retinal ganglion cell axon regeneration by recapitulating developmental mechanisms: effects of recruitment of the mTOR pathway
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (106 cells/ml) in Sf-900 III SFM Medium supplemented with 12.5 U/ml penicillin/streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27°C and 130 rpm for 2-3 days. .. Amplified viruses were purified and concentrated by ultracentrifugation of 27 ml virus-containing supernatant underlaid with 2.7 ml sucrose solution (25% sucrose with 5 mM NaCl and 10 mM EDTA in H 2 O) in OptiSeal polypropylene tubes (Beckmann Coulter) at 80,000 g and 4°C for 80 min.

Article Title: Targeting Vasohibins to Promote Axon Regeneration
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher Scientific) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (106 cells/ml) in Sf-900 III SFM Medium (Thermo Fisher Scientific) supplemented with 12.5 U/ml penicillin/ streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27°C and 110 rpm for 4 d. Baculovirus preparations were pretested on AAV293 cells overnight by adding 1% (v/v) virus. ..

Article Title: Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus.
Article Snippet: .. Suspension cultures of Sf21 insect cells were grown in 125 ml or 250 ml polycarbonate Erlenmeyer flasks with vent cap (CORNING, #431143, #431144) at 27◦C in a shaking incubator. ..

Article Title: Highly efficient transduction of primary adult CNS and PNS neurons
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (10 6 cells/ml) in Sf-900 III SFM Medium supplemented with 12.5 U/ml penicillin/streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27 °C and 130 rpm for 2–3 days. .. Amplified viruses were purified and concentrated by ultracentrifugation of 27 ml virus-containing supernatant underlayed with 2.7 ml sucrose solution (25% sucrose with 5 mM NaCl and 10 mM EDTA in H 2 O) in OptiSeal polypropylene tubes (Beckmann Coulter) at 80,000 g and 4 °C for 80 min.

Virus:

Article Title: VASH1/2 inhibition accelerates functional recovery of injured nerves
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (10 6 cells/ml) in Sf-900 III SFM Medium supplemented with 12.5 U/ml penicillin/streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27°C and 110 rpm for 4 days. ..

Article Title: Human retinal ganglion cell axon regeneration by recapitulating developmental mechanisms: effects of recruitment of the mTOR pathway
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (106 cells/ml) in Sf-900 III SFM Medium supplemented with 12.5 U/ml penicillin/streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27°C and 130 rpm for 2-3 days. .. Amplified viruses were purified and concentrated by ultracentrifugation of 27 ml virus-containing supernatant underlaid with 2.7 ml sucrose solution (25% sucrose with 5 mM NaCl and 10 mM EDTA in H 2 O) in OptiSeal polypropylene tubes (Beckmann Coulter) at 80,000 g and 4°C for 80 min.

Article Title: Targeting Vasohibins to Promote Axon Regeneration
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher Scientific) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (106 cells/ml) in Sf-900 III SFM Medium (Thermo Fisher Scientific) supplemented with 12.5 U/ml penicillin/ streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27°C and 110 rpm for 4 d. Baculovirus preparations were pretested on AAV293 cells overnight by adding 1% (v/v) virus. ..

Article Title: Highly efficient transduction of primary adult CNS and PNS neurons
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (10 6 cells/ml) in Sf-900 III SFM Medium supplemented with 12.5 U/ml penicillin/streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27 °C and 130 rpm for 2–3 days. .. Amplified viruses were purified and concentrated by ultracentrifugation of 27 ml virus-containing supernatant underlayed with 2.7 ml sucrose solution (25% sucrose with 5 mM NaCl and 10 mM EDTA in H 2 O) in OptiSeal polypropylene tubes (Beckmann Coulter) at 80,000 g and 4 °C for 80 min.

Incubation:

Article Title: VASH1/2 inhibition accelerates functional recovery of injured nerves
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (10 6 cells/ml) in Sf-900 III SFM Medium supplemented with 12.5 U/ml penicillin/streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27°C and 110 rpm for 4 days. ..

Article Title: Human retinal ganglion cell axon regeneration by recapitulating developmental mechanisms: effects of recruitment of the mTOR pathway
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (106 cells/ml) in Sf-900 III SFM Medium supplemented with 12.5 U/ml penicillin/streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27°C and 130 rpm for 2-3 days. .. Amplified viruses were purified and concentrated by ultracentrifugation of 27 ml virus-containing supernatant underlaid with 2.7 ml sucrose solution (25% sucrose with 5 mM NaCl and 10 mM EDTA in H 2 O) in OptiSeal polypropylene tubes (Beckmann Coulter) at 80,000 g and 4°C for 80 min.

Article Title: Targeting Vasohibins to Promote Axon Regeneration
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher Scientific) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (106 cells/ml) in Sf-900 III SFM Medium (Thermo Fisher Scientific) supplemented with 12.5 U/ml penicillin/ streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27°C and 110 rpm for 4 d. Baculovirus preparations were pretested on AAV293 cells overnight by adding 1% (v/v) virus. ..

Article Title: Highly efficient transduction of primary adult CNS and PNS neurons
Article Snippet: In brief, recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock. .. Baculoviruses were amplified by inoculation of 50 ml Sf9 suspension cultures (10 6 cells/ml) in Sf-900 III SFM Medium supplemented with 12.5 U/ml penicillin/streptomycin (Biochrom) in 125 ml polycarbonate Erlenmeyer flasks with vent cap (Corning) with 1 ml virus stock solution and incubation at 27 °C and 130 rpm for 2–3 days. .. Amplified viruses were purified and concentrated by ultracentrifugation of 27 ml virus-containing supernatant underlayed with 2.7 ml sucrose solution (25% sucrose with 5 mM NaCl and 10 mM EDTA in H 2 O) in OptiSeal polypropylene tubes (Beckmann Coulter) at 80,000 g and 4 °C for 80 min.

Formulation:

Article Title: Preclinical evaluation of manufacturable SARS-CoV-2 spike virus-like particles produced in Chinese Hamster Ovary cells
Article Snippet: .. Cells were maintained in a proprietary media formulation in polycarbonate Erlenmeyer flasks with 0.2 μm vent cap (Corning) with constant orbital shaking at 120 rpm in a humidified incubator with 5% CO 2 at 37 °C. ..

Article Title: Preclinical evaluation of manufacturable SARS-CoV-2 spike virus-like particles produced in Chinese Hamster Ovary cells.
Article Snippet: .. Cells were maintained in a proprietary media formulation in polycarbonate Erlenmeyer flasks with 0.2 μm vent cap (Corning) with constant orbital shaking at 120 rpm in a humidified incubator with 5% CO2 at 37 °C. ..

Incubator:

Article Title: Preclinical evaluation of manufacturable SARS-CoV-2 spike virus-like particles produced in Chinese Hamster Ovary cells
Article Snippet: .. Cells were maintained in a proprietary media formulation in polycarbonate Erlenmeyer flasks with 0.2 μm vent cap (Corning) with constant orbital shaking at 120 rpm in a humidified incubator with 5% CO 2 at 37 °C. ..

Article Title: Preclinical evaluation of manufacturable SARS-CoV-2 spike virus-like particles produced in Chinese Hamster Ovary cells.
Article Snippet: .. Cells were maintained in a proprietary media formulation in polycarbonate Erlenmeyer flasks with 0.2 μm vent cap (Corning) with constant orbital shaking at 120 rpm in a humidified incubator with 5% CO2 at 37 °C. ..



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